Original Research

Preliminary DNA fingerprinting of the turf grass Cynodon dactylon (Poaceae: Chloridoideae)

R. Roodt, J. J. Spies, T. H. Burger
Bothalia | Vol 32, No 1 | a474 | DOI: https://doi.org/10.4102/abc.v32i1.474 | © 2002 R. Roodt, J. J. Spies, T. H. Burger | This work is licensed under CC Attribution 4.0
Submitted: 10 September 2002 | Published: 11 September 2002

About the author(s)

R. Roodt, Department of Plant Sciences, South Africa
J. J. Spies, Department of Plant Sciences, South Africa
T. H. Burger, Department of Plant Sciences, South Africa

Full Text:

PDF (1MB)

Abstract

Identification of different cultivars of turf grasses is often very difficult. In a preliminary attempt to identify different cultivars o f Cynodon dactylon (L.) Pers.. random amplified polymorphic DNA (RAPD) analyses of some well-known cultivars used in South Africa, i.e. Bayview. Cape Royal, Florida. Hamsmith. Silverton Blue. Skaapplaas and Titdwart. as well as 10 potential new cultivars, were done. These results were used to determine the genetic distances among cultivars. Only five primers w ere needed to obtain a specific fragment pattern for each cultivar. The degree o f amplification w as used as an additional criterion by including all visible fragments, excluding very faint fragments and only including the brightest fragments. The neighbour-joining trees o f C.  dactylon showed best resolution from the data set w ith all visible fragments included. although fragment intensity did not affect the tree topology. The cultivars Silverton Blue and Bayview exhibited the greatest genetic variation and two potential new cultivars were identified. RAPD analyses can. therefore, be used to distin­guish between different C. dactylon cultivars and to determine the genetic variation between them by calculating genetic distances.

Keywords

<i>Cynodon</i> Rich., genetic distances, identification. RAPDs, turf grasses

Metrics

Total abstract views: 2224
Total article views: 2515

 

Crossref Citations

1. Intra-population genetic diversity of Buchloe dactyloides (Nutt.) Engelm (buffalograss) determined using morphological traits and sequence-related amplified polymorphism markers
Feifei Wu, Junhan Chen, Jianli Wang, Xianguo Wang, Yan Lu, Yaming Ning, Yongxiang Li
3 Biotech  vol: 9  issue: 3  year: 2019  
doi: 10.1007/s13205-019-1632-9